Institute for Integrated Cell-Material Sciences, Kyoto University (iCeMS) iCeMS

Carlton, Peter PI Lab
Meiosis, Chromosome Biology, Optical Microscopy

Member

Carlton, Peter Associate Professor Carlton, Peter
Kafer, Georgia Research Associate  
Li, Xuan Research Associate  
Rillo, Regina Research Associate  
Sato, Aya Research Associate  
Chartrand, Stephane Research Support Staff  
Fujita, Yoko Research Support Staff  
Li, Hao Research Support Staff  
Nakamura, Chihiro Research Support Staff  
Tanaka, Yoshihisa Research Support Staff  
Yamauchi, Yuji Research Support Staff  
Nishimura, Makiko Secretary  

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Research Overview

Our research group studies how the structure and dynamic behavior of chromosomes in meiosis control pairing, recombination, and the correct transmission of the genome. Errors in meiosis cause many human health problems, from infertility to birth defects. We use the nematode Caenorhabditis elegans as a model system for its excellent genetic and cytological qualities. A main focus of the lab is the use of superresolution techniques such as 3D structured illumination and single-molecule composition microscopy to assess chromosome structure at the mesoscale. We aim to find the mechanisms underlying the recognition of homology, and understand why meiotic recombination occurs between homologs rather than sister chromatids. An additional area of interest is the study of dynamic processes such as chromosome movement with fast three-dimensional multiwavelength fluorescence imaging under conditions that preserve full viability. Collaborative efforts within iCeMS apply advanced optical microscopy to questions of nuclear organization in pluripotent stem cells, meiosis in mammalian systems, and neural development.


Fig. 1: Meiosis creates haploid gametes from diploid precursors by an intricately coordinated reorganization of chromosomes.
Enlarge Fig. 1: Meiosis creates haploid gametes from diploid precursors by an intricately coordinated reorganization of chromosomes.
Fig. 2: Three-dimensional Structured Illumination Microscopy (3D-SIM) doubles the resolution limit of optical microscopy.
Enlarge Fig. 2: Three-dimensional Structured Illumination Microscopy (3D-SIM) doubles the resolution limit of optical microscopy.
Fig. 3: 3D-SIM image of meiotic chromosomes of corn (Zea mays) at the pachytene stage, when all homologous chromosomes are paired, synapsed, and undergoing recombination. The DNA (magenta) is arranged radially around the synaptonemal complex (cyan), which is visible as two parallel strands, separated by ~190nm, twisting around each other.
Enlarge Fig. 3: 3D-SIM image of meiotic chromosomes of corn (Zea mays) at the pachytene stage, when all homologous chromosomes are paired, synapsed, and undergoing recombination. The DNA (magenta) is arranged radially around the synaptonemal complex (cyan), which is visible as two parallel strands, separated by ~190nm, twisting around each other.

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    Selected papers

  • Carlton, P. M. Application of advanced fluorescence microscopy to the structure of meiotic chromosomes. Biophys. Rev. DOI: 10.1007/s12551-013-0116-0 (2013).
  • Carlton, P. M., Boulanger J., Kervrann C., Sibarita J. B., Salamero J., Gordon-Messer S., Bressan D., Haber J. E., Haase S., Shao L., Winoto L., Matsuda A., Kner P., Uzawa S., Gustafsson M., Kam Z., Agard D. A., Sedat J. W. Fast live simultaneous multiwavelength four-dimensional optical microscopy. Proc. Natl. Acad. Sci. USA 107, 16016-16022 (2010).
  • Schermelleh, L.*,Carlton, P. M.*,Haase, S., Shao, L., Winoto, L., Kner, P., Burke, B., Cardoso, M. C., Agard, D. A., Gustafsson, M. G., Leonhardt, H. and Sedat, J. W. Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science 320, 1332-1336 (2008).
*co-first authors

Contact

Email carlton-g@icems.kyoto-u.ac.jp
Phone 075-753-9853
Website Open new windowCarlton Lab

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