Stem-Cell Biology, Developmental Biology
|Aiba, Kazuhiro||Associate Professor|
|Miyazaki, Takamichi||Assistant Professor|
|Ayuzawa, Rie||Research Associate|
|Yanagawa, Nariaki||Research Associate|
|Zhang, Haixin||Research Associate|
|Fujikawa, Saori||Research Support Staff|
|Isobe, Takehisa||Research Support Staff|
|Tachikawa, Naoko||Research Support Staff|
|Tanikawa, Maki||Research Support Staff|
|Uda, Junki||Research Support Staff|
Our research group has been working on development and differentiation of embryonic stem cells and germ cells in mammals. In particular, we have established mouse, cynomolgus monkey, and human embryonic stem (ES) cell lines, and we have been carrying out various aspects of basic and application research using pluripotent stem cells, including human ES and iPS cells.
We have developed methods of genetic modification in primate and human pluripotent stem cells, including conditional expression such as the Tet-On/Off system, expression of multiple transgenes, and the homologous recombination method. More recently, our group has created normal and disease model cells for disease mechanism research and drug discovery tools, which are important applications of pluripotent stem cell lines. These include production of neurodegenerative disease model cells by introduction of mutated genes, toxicology studies using cardiomyocytes, and chemical screening for stem cell control.
- Creation and analysis of model cells from human ES and iPS cell lines. They include neurodegenerative disease model cells, such as Alzheimer, ALS and Huntington disease models, which are produced by genetic modification of stem cell lines and differentiation into relevant cells in each disease. Production of abnormal protein/peptides and disease mechanisms will be examined in collaboration with other research groups in the iCeMS.
- Control of stem cells with chemical compounds and nano/meso/micro-fabricated materials for growth and differentiation of ES/iPS cells in collaboration with chemical biology groups (such as the Uesugi and Sugiyama Lab) and nano/meso/micro-engineering groups (such as the Chen Lab). For example, we have identified novel small molecules which can induce efficient and robust cardiomyocyte differentiation from many human ES and iPS cell lines in totally defined xeno-free conditions.
- Development of novel technologies for large-scale production of high-quality human pluripotent stem cells using 3D culture system. It is a government-supported project for medical and pharmaceutical application of stem cells, and carried out by collaboration with several high-technology companies in addition to the collaboration with many academic research groups.
- Minami, I., Yamada, K., Otsuji, T. G., Yamamoto, T., Shen, Y., Otsuka, S., Kadota, S., Morone, N., Barve, M., Asai, Y., Tenkova-Heuser, T., Heuser, J. E., Uesugi, M., Aiba, K. and Nakatsuji, N. A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep. 2, 1448-1460 (2012).
- Kadota, S., Minami, I., Morone, N., Heuser, J. E., Agladze, K. and Nakatsuji, N. Development of a reentrant arrhythmia model in human pluripotent stem cell-derived cardiac cell sheets. Eur. Heart J. DOI: 10.1093/eurheartj/ehs418 (2012).
- Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K. and Kawase, E. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3 DOI:10.1038/ncomms2231 (2012).
- Wada, T., Goparaju, S. K., Tooi, N., Inoue, H. Takahashi, R., Nakatsuji, N. and Aiba, K. Amyotrophic lateral sclerosis model derived from human embryonic stem cells overexpressing mutant superoxide dismutase 1. Stem Cells Transl. Med. 1, 396-402 (2012).
- The International Stem Cell Initiative: Andrews, P. W. and others (incl. Miyazaki, T., Nakatsuji, N., Suemori, H., Takahashi, K., Yamanaka, S.) Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nat Biotechnol. 29, 1132-1144 (2011).
|Website||Nakatsuji Lab (Department of Development and Differentiation)
Nakatsuji Lab (Laboratory of embryonic stem cell research)