- July 13, 2017
iCeMS Furukawa Lab Seminar: Dr Cristina FlorsAugust 9, 2017
Dr Cristina Flors
IMDEA Nanoscience Institute, Spain
Novel correlative microscopy tools to study biology and biomaterials at the nanoscale
Correlative microscopy is a powerful approach that combines the capabilities of individual microscopy techniques, typically with the aim of obtaining high chemical specificity and detailed structural information of the same sample area. In this talk, I will present our most recent progress in developing novel tools for different modalities of correlative imaging. The first part will deal with the implementation of a novel correlative microscope that allows sequential imaging of the same sample area by atomic force microscopy (AFM) and super-resolution fluorescence microscopy . I will discuss the technical aspects of the correlative microscope, its application to validate and scrutinize super-resolution methods, and its use to characterize hybrid bionanomaterials . In the second part of the talk, the screening and characterization of fluorescent proteins as genetically-encoded tags for correlative light and electron microscopy (CLEM) will be presented [3,4]. These fluorescent proteins produce reactive oxygen species upon irradiation, which locally photooxidize diaminobenzidine to form an osmiophilic precipitate that gives contrast in EM. Both correlative imaging modalities have the power to answer new biological questions at the nanoscale.
-  Bondia, P; Jurado, R; Casado, S; Domínguez-Vera, J M; Gálvez, N; Flors, C, Small 2017, 13, 1603784.
-  Ruiz-González, R; Cortajarena, A L; Mejias, S H; Agut, M; Nonell, S; Flors, C, J Am Chem Soc 2013, 135, 9564.
-  Rodríguez-Pulido, A; Cortajarena, A L; Torra, J; Ruiz-González, R; Nonell, S; Flors, C, Chem Commun 2016, 52, 8405.
|Date/Time||August 9, 2017 / 15:00-16:30
|Venue||Kyoto University KUIAS/iCeMS Main Building 2F Seminar Room (A207)
|Host||Furukawa Lab, Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University Institute for Advanced Study|
|Contact||iCeMS Furukawa Lab
furukawa-g [at] icems.kyoto-u.ac.jp