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  • iCeMS Furukawa Lab Seminar: Dr. Cristina Flors

iCeMS Furukawa Lab Seminar: Dr. Cristina Flors

August 9, 2017

Dr. Cristina Flors

IMDEA Nanoscience Institute, Spain

Novel correlative microscopy tools to study biology and biomaterials at the nanoscale

Correlative microscopy is a powerful approach that combines the capabilities of individual microscopy techniques, typically with the aim of obtaining high chemical specificity and detailed structural information of the same sample area. In this talk, I will present our most recent progress in developing novel tools for different modalities of correlative imaging. The first part will deal with the implementation of a novel correlative microscope that allows sequential imaging of the same sample area by atomic force microscopy (AFM) and super-resolution fluorescence microscopy [1]. I will discuss the technical aspects of the correlative microscope, its application to validate and scrutinize super-resolution methods, and its use to characterize hybrid bionanomaterials [2]. In the second part of the talk, the screening and characterization of fluorescent proteins as genetically-encoded tags for correlative light and electron microscopy (CLEM) will be presented [3,4]. These fluorescent proteins produce reactive oxygen species upon irradiation, which locally photooxidize diaminobenzidine to form an osmiophilic precipitate that gives contrast in EM. Both correlative imaging modalities have the power to answer new biological questions at the nanoscale.

    [1] Monserrate, A.; Casado, S.; Flors, C, ChemPhysChem 2014, 15, 647.
  • [2] Bondia, P; Jurado, R.; Casado, S.; Domínguez-Vera, J. M.; Gálvez, N.; Flors, C, Small 2017, 13, 1603784.
  • [3] Ruiz-González, R.; Cortajarena, A. L.; Mejias, S. H.; Agut, M.; Nonell, S.; Flors, C, J. Am. Chem. Soc. 2013, 135, 9564.
  • [4] Rodríguez-Pulido, A.; Cortajarena, A. L.; Torra, J.; Ruiz-González, R.; Nonell, S.; Flors, C., Chem. Commun. 2016, 52, 8405.
Date/Time August 9, 2017 / 15:00-16:30
Venue Kyoto University KUIAS/iCeMS Main Building 2F Seminar Room (A207)
Access map
Admission Free
Registration Not required
Language English
Host Furukawa Lab, Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University Institute for Advanced Study
Contact iCeMS Furukawa Lab
furukawa-g [at]

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