第48回 アイセムスセミナー: Moon-IL Kang 博士

Transcription factor AP-1 activity is required both to induce and to maintain tumor phenotype in carcinogenesis. The high throughput cell-based assay is designed to screen compound targeting AP-1 by using FRET (Fluorescence Resonance Energy Transfer) substrate to quantify the activity of β-lactamase reporter. Synthetic compound libraries and the National Cancer Institute natural product extract were screened to identify AP-1 hit, and the optimized screening was coupled with cytotoxicity assay in the same wells without having to do it in parallel. Several natural and synthetic compounds have been confirmed they could be promising candidates as anti-cancer drug having potential therapeutic activity for cancer treatment or prevention.

講演者 Moon-IL Kang, Ph.D.
Laboratory of Cancer Prevention, Center for Cancer Research
National Cancer Institute at Frederick (NCI-Frederick)
米国 国立衛生研究所 (NIH)
演題 A High Throughput Cell-Based Assay for Identifying Inhibitors of AP-1 from NCI Compound Repositories
日時 2010年3月31日(水)16:30-17:30
場所 京都大学 iCeMSコンプレックス1 本館 2階 セミナールーム (A207)
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フライヤー PDF FilePDF (140KB)
主催 京都大学 物質-細胞統合システム拠点(iCeMS=アイセムス)
連絡先 京都大学iCeMS植田グループ
ueda-g@icems.kyoto-u.ac.jp