第50回 アイセムスセミナー: 長谷川 光一 博士

The application of human pluripotent stem cells in regenerative medicine will require the propagation of large numbers of cells in the absence of animal products (xeno-free conditions). To date, most xeno-free culture systems require human feeder cells and/or highly complicated culture media. Particularly, to meet good manufacturing practice (GMP) standards, replacing such components with small molecules would provide significant advantages. Replacement with small molecules is contingent upon our understanding of the key signaling pathways involved in human pluripotent self- renewal. We performed by comparative microarray analysis of normal human embryonic stem cells (hESCs) to diploid hESC sub-lines that had been adapted to grow as single cells. Based on the analysis, we investigated several small molecules involved in the signaling cascades that enhance hESC survival and/or self-renewal. We found that a combination of Wnt ligands and a small molecule that modulates Wnt-mediated transcription could support hESC self-renewal and survival. Based on this discovery, we developed a novel xeno-free, feeder-free simple human pluripotent stem cell culture system consisting of a few defined growth factors: insulin, bFGF and Wnt3a, plus the small molecule Wnt modulator in DMEMIF-12 medium. Our culture conditions do not include complicated supplements, serum, serum replacement or albumin. In addition, we also developed a chemically-defined medium that could induce definitive endodermal cells from hESC efficiently.

講演者 長谷川 光一 博士
Assistant Professor of Research
Department of Call and Neurobiology
Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research
米国 南カリフォルニア大学 Keck School of Medicine
演題 Control of Self-Renewal and Differentiation of Human Pluripotent Stem Cells by Small Molecules
日時 2010年4月6日(木)16:00-17:00
場所 京都大学 iCeMSコンプレックス1 本館 2階 セミナールーム (A207)
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主催 京都大学 物質-細胞統合システム拠点(iCeMS=アイセムス)
連絡先 京都大学iCeMS中辻グループ
nakatsuji-g@icems.kyoto-u.ac.jp