第64回 アイセムスセミナー / CeMI セミナーシリーズ 19: 谷口 雄一 博士

CeMI: Center for Meso-Bio Single-Molecule Imaging

Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic bacterial populations. However, these molecules often exist in low copy numbers and are difficult to detect in single cells. We carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli. We found that almost all protein number distributions can be described by the gamma distribution with two fitting parameters which, at low expression levels, have clear physical interpretations as the transcription rate and protein burst size. At high expression levels, the distributions are dominated by extrinsic noise. We found that a single cell's protein and mRNA copy numbers for any given gene are uncorrelated.

Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, Hearn J, Emili A, Xie XS. Science 329, 533-538 (2010)

講演者	谷口 雄一 博士 Department of Chemistry and Chemical Biology ハーバード大学
演題	Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
日時	2010年11月30日（火）14:00-15:30
場所	京都大学 iCeMSコンプレックス1 本館 2階 セミナールーム (A207) アクセスマップ
フライヤー	PDF (148KB)
主催	京都大学 物質－細胞統合システム拠点（iCeMS＝アイセムス）
連絡先	iCeMS 原田グループ harada-g@icems.kyoto-u.ac.jp