第116回 アイセムスセミナー / CeMI セミナーシリーズ 33: Prof. Kai Johnsson, Assoc Prof. Scott Blanchard, Prof. Ikonen, Assoc. Prof. Derek Toomre
CeMI：Center for Meso-Bio Single-Molecule Imaging
Prof. Kai Johnsson
Visualizing biochemical activities in living cells
The visualization and characterization of biologically relevant molecules and activities inside
living cells continues to transform cell biology into a truly quantitative science. However, despite the spectacular achievements in some areas of cell biology, the majority of cellular processes still operate invisibly, not illuminated by even our brightest laser beams. Further progress will therefore not only depend on improvements in instrumentation but increasingly on the development of new (fluorescent) sensors and other synthetic probes to target and characterize these activities. The research conducted by Dr. Johnsson addresses these needs by developing and applying chemical approaches to observe and manipulate protein function in living cells. In this talk, Dr. Johnsson will discuss (i) the design of new fluorescent probes and their applications in biology, (ii) chemical tools to study centrosome biology, as well as (iii) semisynthetic fluorescent sensor proteins.
Assoc. Prof. Scott C. Blanchard
Understanding regulation in biological systems through single-molecule imaging
Dr. Blanchard has employed a battery of biophysical tools, including single-molecule
fluorescence and fluorescence resonance energy transfer imaging, in order to gain new insights into the fundamentally dynamic nature of biological systems. Two, highly conserved biological processes remain the principle model systems investigated by his lab: ribosome-catalyzed protein synthesis and neurotransmitter-sodium symporter transport proteins that facilitate the movement of solute molecules across cellular envelope. Progress towards Dr. Blanchard's long-term goals of obtaining a deeper, quantitative understanding of how biological systems are regulated in the cell and directly imaging single-molecules in real time within living cells will be discussed.
Prof. Elina Ikonen
Cellular trafficking of low-density lipoprotein derived lipids: insights from imaging
Dr. Ikonen will discuss cholesterol and sphingolipid trafficking upon entry into the endocytic route via LDL-receptor mediated uptake. These include aspects of 1) lipid flow for recycling vs. degradation, 2) post-degradative exit of endo-lysosomal cholesterol and sphingolipids, and 3)
delivery of degradation products to the plasma membrane.
Assoc. Prof. Derek Toomre
Spatial Control of Exocytosis:
New nanoscopes and analysis to 'connect the dots'
A main focus of Dr. Toomre's talk will be the application on state of the art super-resolution
microscopes (TIRFM, PALM, STED, SIM) to see cellular processes (e.g. cytoskeleton, membrane traffic) at an unprecedented resolution. A challenge, however, is to know how to connect the dots when analyzing spatial point processes so as to tell in an unbiased manner if they are 'linked' (same object) or correlated (same region). Herein, Dr. Toomre will discuss methods to see and control the dots (exo/endocytic events) and novel spatial statistics methods to quantify them. This reveals surprising spatial 'hotspots' and 'zones of exclusion' in cells and their potential significance will be discussed.
| 講演者 | Prof. Kai Johnsson École Polytechnique Fédérale de Lausanne スイス Assoc. Prof. Scott C. Blanchard Weill Cornell Medical College 米国 Prof. Elina Ikonen Institute of Biomedicine, Faculty of Medicine, University of Helsinki フィンランド Assoc. Prof. Derek Toomre Department of Cell Biology, Yale University School of Medicine 米国 |
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| 日時 | 2012年8月29日（水）12:50-17:10 |
| 場所 | 京都大学 iCeMS 本館（#71）2階 セミナールーム (A207) アクセスマップ |
| フライヤー |  PDF (598KB) |
| 主催 | 京都大学 物質－細胞統合システム拠点（iCeMS＝アイセムス） |
| 共催 | 京都大学医学研究科グローバルCOEプログラム「生命原理の解明を基とする医学研究教育拠点」 |
| 連絡先 | iCeMS 楠見グループ akusumi@frontier.kyoto-u.ac.jp | fax 075-751-4113 |