第118回 アイセムスセミナー: Prof. Yuh-Lin Wang

Detecting bacteria in clinical samples without the time-consuming culture process is most desired for rapid diagnosis. Such a culture-free detection needs to capture and analyze bacteria from a body fluid usually containing complicate constituents. Here we show that vancomycin (Van) coating of a special substrate with arrays of Ag-nanoparticles, which can provide label-free analysis of bacteria via surface enhanced Raman spectroscopy (SERS), leads to 1000 folds increase in its capability to capture bacteria without introducing significant spectral interference. Bacteria spiked in human blood can be concentrated onto a microscopic Van-coated area while blood cells are excluded. Furthermore, A Van-coated substrate provides distinctly different SERS spectra of Van-susceptible and Van-resistant Enterococcus, indicating its potential use for drug-resistance test. Our results represent a critical step towards the creation of SERS-based multifunctional biochips for rapid culture/label-free detection and drug-resistant testing of microorganisms in clinical samples.

講演者 演題	Prof. Yuh-Lin Wang 台湾 中央研究院 原子分子科学研究所 演題：Functionalized Arrays of Raman-Enhancing Nanoparticles for Capture and Culture-Free Analysis of Bacteria in Human Blood
日時	2012年9月28日（金）10:30-11:30
場所	京都大学 iCeMS 本館（#71）2階 セミナールーム (A207) アクセスマップ
フライヤー	PDF (93KB)
主催	京都大学 物質－細胞統合システム拠点（iCeMS＝アイセムス）
共催	京都大学医学研究科グローバルCOEプログラム「生命原理の解明を基とする医学研究教育拠点」
連絡先	iCeMS 髙野グループ takano-g@icems.kyoto-u.ac.jp