Halting iPS cell reprogramming early leads to cancer

February 17, 2014


Yasuhiro Yamada

A research team led by CiRA and iCeMS Professor Yasuhiro Yamada's group has discovered that, when cells are subjected to incomplete reprogramming in vivo, epigenetic modifications take place which promote carcinogenesis. The findings of the research by the group including postgraduate student Kotaro Onishi (Kyoto University, CiRA/Gifu University Graduate School) and researcher Katsunori Semi (Kyoto University CiRA/iCeMS) were published on February 13, 2014 (U.S. Eastern time) in the U.S. scientific journal Cell.

iPS cells and cancer cells have the common attribute of being capable of unlimited proliferation, but whereas cancer is thought to arise through a series of gene mutations, iPS cells are generated by reprogramming somatic cells, which need not involve any gene mutation.

However, when mouse cells were exposed in vivo to four reprogramming factors (Oct3/4, Sox2, Klf4, c-Myc) in such a way as to cause incomplete reprogramming, a major change occurred in the DNA methylation pattern (epigenome), resulting in tumorigenesis in a range of tissues. Tumors arising in the kidney through this process were very similar to Wilms' tumor, a pediatric kidney cancer, in terms of histological and molecular biological features. An examination of the cells of these tumors revealed no gene mutation, but did identify modifications in the epigenome, which was found to have altered to a pattern resembling that of pluripotent stem cells. Furthermore, the researchers were able to generate normal kidney cells from iPS cells created by reprogramming the tumor cells. These findings indicate the possibility that epigenetic regulation may promote tumorigenesis in certain types of cancer.

The findings of the research demonstrated that incomplete reprogramming using a procedure devised to reprogram mouse cells in vivo leads to the formation of tumors resembling nephroblastoma. Previously it had been thought that cumulative gene mutations were important in oncogenesis. However, the results of the present research indicate that, with certain types of tumor, cancer can be triggered not by gene mutation, but by epigenetic alterations. In other words, if it were possible to alter epigenetic conditions, it might be possible to alter the properties of cancer cells, which could lead in the future to new cancer therapies.

The present research additionally made use of iPS cell technology to develop a technique for epigenetic regulation that does not induce genomic mutation. It is hoped that using iPS cell technology in this way will bring new perspectives to disease research.

Publication Information

Extenal LinkPremature Termination of Reprogramming In Vivo Leads to Cancer Development through Altered Epigenetic Regulation
Kotaro Ohnishi1,2*, Katsunori Semi1,3*, Takuya Yamamoto1,3, Masahito Shimizu2, Akito Tanaka1, Kanae Mitsunaga1, Keisuke Okita1, Kenji Osafune1, Yuko Arioka1, Toshiyuki Maeda4, Hidenobu Soejima4 Hisataka Moriwaki 2, Shinya Yamanaka 1,3,5, Knut Woltjen 1,6 & Yasuhiro Yamada1,3,7

Cell | Published 13 February 2014 | DOI: http://dx.doi.org/10.1016/j.cell.2014.01.005

  1. Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan
  2. Department of Medicine, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
  3. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8507, Japan
  4. Division of Molecular Genetics and Epigenetics, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga 849-8501, Japan
  5. Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA
  6. Hakubi Center for Advanced Research, Kyoto University, Kyoto 606-8507, Japan
  7. PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, 332-0012, Japan

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