17th iCeMS Seminar - Prof. Dr. Dietrich Keppler

Transport proteins mediating the uptake and efflux of small molecules across cellular membrane domains are key determinants of the absorption, disposition, and elimination of many drugs and endogenous substances. Cell lines stably expressing uptake and/or efflux transporters demonstrate the decisive role of transport proteins in the vectorial transport of many organic anions, organic cations, and neutral compounds across polarized epithelial cells. In vitro systems for studies on vectorial transport of the cationic berberine include double-transfected polarized cells permanently expressing the human organic cation transporter 1 (OCT1, SLC22A1) together with the efflux pump MDR1 P glycoprotein (ABCB1). Cells stably expressing OATP1B3 as uptake transporter together with the efflux pump MRP2 (ABCC2) served in the data-based mathematical modeling of vectorial transport of the anionic bromosulfophthalein and resulted in a quantitative description of this process and its components.

Lecturer	Prof. Dr. Dietrich Keppler German Cancer Research Center
Title	Vectorial Transport of Small Molecules Across Cellular Membranes by Uptake and Efflux Transport Proteins
Date / Time	March 19, 2009 / 15:30-16:30
Venue	Conference Room, 2nd Floor of the West Wing, iCeMS Complex 1 Directions
Flyer	PDF (743 KB)
Held by	Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University
Contact	Ueda Lab at ueda-g@icems.kyoto-u.ac.jp