34th iCeMS Seminar/Commemorating the Establishment of the Center for Meso-Bio Single-Molecule Imaging (CeMI) 9: Jacco van Rheenen Ph.D. and Dinah Loerke Ph.D.
CeMI：Center for Meso-Bio Single-Molecule Imaging
Cofilin-Mediated Actin Remodeling is Important for Migration and Metastasis of Mammary Carcinoma Cells
(Jacco van Rheenen, Ph.D.)
Cofilin is an actin severing protein, involved in cell migration and metastasis. Using intravital and high resolution imaging, Drs. Jacco van Rheenen, John Condeelis, and their colleagues showed that cofilin gets locally released from the membrane and activated upon phospholipase C-mediated PIP2 hydrolysis. Based on these and other observations, they conclude that these processes that occur at the cell cortex is a critical step for initiating EGF-induced migration and metastasis.
Correlative Intensity Timecourse Analysis in Endocytosis
(Dinah Loerke, Ph.D.)
Using total internal reflection fluorescence microscopy (TIR-FM) and automated detection-tracking, Drs. Dinah Loerke, Gaudenz Danuser, and their colleagues have measured the maturation dynamics of large populations of clathrin-coated pits (CCPs). While fluorescence intensity timecourses of clathrin and other coat proteins contain valuable information, intensity analysis is far from trivial due to the high noise. They have developed an intensity analysis assay that retains heterogeneity between different lifetime CCP cohorts, and preserves fast dynamics within the cohort, clarifying the association of endocytic factors with cortical actin dynamics across the entire CCP lifetime.
| Lecturers & Titles |
Jacco van Rheenen, Ph.D. Assistant Professor, Department of Physics & Astronomy University of Denver, U.S.A. "Correlative Intensity Timecourse Analysis in Endocytosis" |
|---|---|
| Date / Time | Friday, October 23, 2009 / 15:00-17:00 |
| Venue | The 1st Meeting Room 1F of the West Building Institute for Frontier Medical Sciences  Directions |
| Flyers |  PDF (576KB) |
| Sponsors | Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University The Institute for Frontier Medical Sciences, Kyoto University Membrane Mechanisms Project, ICORP-JST |
| Contact | Aki Kusumi, iCeMS fax: 075-751-4113 e-mail: akusumi@frontier.kyoto-u.ac.jp |