64th iCeMS Seminar/CeMI Seminar Series 19: Dr. Yuichi Taniguchi

CeMI: Center for Meso-Bio Single-Molecule Imaging

Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic bacterial populations. However, these molecules often exist in low copy numbers and are difficult to detect in single cells. We carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli. We found that almost all protein number distributions can be described by the gamma distribution with two fitting parameters which, at low expression levels, have clear physical interpretations as the transcription rate and protein burst size. At high expression levels, the distributions are dominated by extrinsic noise. We found that a single cell's protein and mRNA copy numbers for any given gene are uncorrelated.

Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, Hearn J, Emili A, Xie XS. Science 329, 533-538 (2010)

Lecturer	Dr. Yuichi Taniguchi Department of Chemistry and Chemical Biology Harvard University
Title	Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells
Date / Time	November 30, 2010 / 14:00-15:30
Venue	2nd floor Seminar Room (#A207), Main Building iCeMS Complex 1, Kyoto University Directions
Flyer	PDF (136KB)
Host	Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University
Contact	iCeMS Harada Lab harada-g@icems.kyoto-u.ac.jp