155th iCeMS Seminar: Prof. Jean-Jacques Toulmé
Aptamers are oligonucleotides identified in large randomly synthesized libraries containing up to 1015 different oligomers, through in vitro selection, a process known as SELEX (Systematic Evolution of Ligands by EXponantial enrichment). Aptamers have been successfully raised against a wide range of targets: amino acids, nucleic acid bases, proteins, intact viruses and live cells. They generally display a high efficiency of binding thanks to their 3D shape resulting from intramolecular interactions. We raised aptamers against many different target molecules, such as foldamers or biomarkers of human tumors . Following physico-chemical characterization, structural investigation, truncation and optimization these aptamers were converted into biotechnological tools such as probes for imaging brain tumors We also developed methodologies for improving the selection process, a tedious and slow process. The use of in house assembled automated platforms and of functional screening speeds up the identification of aptamers . Aptamers are of high interest for analytical purposes as well as for therapeutic application.
| Lecturer |  ARNA Laboratory, Inserm U869 European Institute of Chemistry and Biology University of Bordeaux |
|---|---|
| Title | Aptamers: a world of structural and functional oligonucleotide diversity |
| Date / Time | Wed, 05 Feb 2014 / 15:00-17:00 |
| Venue | 2nd Floor Seminar Room (#A207) iCeMS Main Building (#70), Kyoto University -> Directions |
| Flyer |  PDF (93KB) |
| Host | Kyoto University Institute for Integrated Cell-Material Sciences (WPI-iCeMS) | Contact | iCeMS Sugiyama Group | sugiyama-g@icems.kyoto-u.ac.jp |