第155回 アイセムスセミナー: Jean-Jacques Toulmé 教授
Aptamers are oligonucleotides identified in large randomly synthesized libraries containing up to 1015 different oligomers, through in vitro selection, a process known as SELEX (Systematic Evolution of Ligands by EXponantial enrichment). Aptamers have been successfully raised against a wide range of targets: amino acids, nucleic acid bases, proteins, intact viruses and live cells. They generally display a high efficiency of binding thanks to their 3D shape resulting from intramolecular interactions. We raised aptamers against many different target molecules, such as foldamers or biomarkers of human tumors . Following physico-chemical characterization, structural investigation, truncation and optimization these aptamers were converted into biotechnological tools such as probes for imaging brain tumors We also developed methodologies for improving the selection process, a tedious and slow process. The use of in house assembled automated platforms and of functional screening speeds up the identification of aptamers . Aptamers are of high interest for analytical purposes as well as for therapeutic application.
| 講演者 |  ARNA Laboratory, Inserm U869 European Institute of Chemistry and Biology University of Bordeaux |
|---|---|
| 演題 | Aptamers: a world of structural and functional oligonucleotide diversity |
| 日時 | 2014年2月5日（水）15:00-17:00 |
| 場所 | 京都大学 iCeMS 本館（#70）2階 セミナールーム (A207) →アクセスマップ |
| フライヤー |  PDF (93KB) |
| 主催 | 京都大学 物質－細胞統合システム拠点（iCeMS＝アイセムス） |
| 連絡先 | 杉山グループ | sugiyama-g@icems.kyoto-u.ac.jp |